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anti fap1α  (Cusabio)


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    Structured Review

    Cusabio anti fap1α
    Anti Fap1α, supplied by Cusabio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fap1%CE%B1/Rabbit+anti-+FAP+Polyclonal+Antibody/pm40426868-68-13-15
    Average 94 stars, based on 1 article reviews
    anti fap1α - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: SIRT1 Mediates the Effects of Sera from Athletes Who Engage in Aerobic Exercise Training in Activating Cells for Wound Healing.
    Article Snippet: The HaCaT, BJ and HUVEC cells were fixed in p-formaldehyde (4% v/v in PBS; Lonza, Basel, Switzerland), permeabilized with Triton X-100 0.4% v/v in PBS (Lonza, Basel, Switzerland) and blocked with goat serum 20% v/v in PBS (Lonza, Basel, Switzerland). .. Next, the cells were incubated with rabbit polyclonal anti-CD31 (1:100; Abcam, Cambridge, UK), anti-FAP1α (1:250; Cusabio, Houston, TX, USA), mouse monoclonal anti-E-cadherin (1:100, Santa Cruz Biotechnologies, Dallas, TX, USA), anti-integrin β1 (1:250; Cell Signaling, Danvers, MS, USA), anti-involucrin (1:100; Santa Cruz Biotechnologies, Dallas, TX, USA) and anti-vinculin (1:100; Santa Cruz Biotechnologies, Dallas, TX, USA) in O/N at 4 ◦C. .. After two washing steps with PBS, the cells were incubated with anti-rabbit and/or anti-mouse Alexa Fluor (488 and/or 555; 1:500; Molecular Probes, Eugene, OR, USA) for 2 h at RT.



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